04-3Development of a method for quality assessment of Japanese Biofango mudtherapy protocol by tensiometric approach / 日本温泉気候物理医学会雑誌

<b>Introduction: </b>The link between the hydration state and the functional effects of formulations and natural systems is been demonstrated by several studies. Measurement of skin hydration has been used to assess barrier function integrity in vivo and stratum corneum (SC), hydration may increase after the topical application of natural or formulate systems. Thermal muds have great hydration properties thanking at its high water contain due to presence of clays minerals¹⁾. Our work was focused on the evaluation of skin’s hydration potentialities of Japanese Biofango^®. <BR><b>Objectives: </b>Mudtherapy increase the hydration state of skin and modify its selective permeability favouring the permeation of therapeutic substances product by maturation process. Our goal was the development of a tensiometric model for assessment and optimisation of Japanese Biofango mudtherapy protocol by the evaluation of skin hydration measuring water contact angles on skin surface²⁾. Our work was performed at Sanraku-en spas centre (Tonami-Japan). <BR><b>Materials and Methods: </b>Biofango was constituted by Kunigel, Kaolinite, and WakuraDiatomite contains montmorillonite. BFM mixture was prepared and collected from Sanraku-en maturation plant. Samples were stored at -25°C. Skin’s hydration state analysis were performed before and after treatment by contact angle method (CA) using DSA 2-Kruss Dynamic Tenskinmeter³⁾ water as liquid test and accordingly to traditional Sanraku-en/Biofango protocol. Four subjects with differents age, sex and weights (KS, YS, MO, and KM) were considered as test. Sanraku-en mudtherapy protocol’s steps were (a) first blood pressure measurement, (b) water’s CA measurements on left and right arm before treatment, (c) thermal bath (8’), (d) water’s CA measurements, (e) BFM mudtherapy (20’), (f) water’s CA measurements on polish skin, (g) shower (3’), (h) water’s CA measurements, and (i) final blood pressure measurement. <BR><b>Results: </b>CA of water on subject KS skin showed elevated levels before (CAt0>89.5 deg) and after (CAtf>89.5 deg) treatment. YS subject showed increases of skin hydration after treatment (CAt0>89.5 deg, CAtf=40.27 deg), MO showed more increase of skin hydration than YS (CAt0>89.5 deg, CAtf=20.12 deg), and KM (CAt0>89.5 deg, CAtf=41.30 deg) showed an hydration state similar to YS. Regarding YS subject, we optimized its mudtherapy by the inversion of bath (CAt0>89.5 deg, CAtf>89.5 deg) with mudtherapy phases. As results a major increase of hydration state respect normal protocol (CAt0>89.5 deg, CAtf =30.4 deg) reflecting on skin moisturize after bath phase also (CAt0>89.5 deg, CAtf=87.43 deg).<BR><b>Conclusions: </b>Analyses of hydration state of skin by measurement of water’s contact angles consented to evaluate the capability of Biofango BFM to modify the selective permeability of stratum corneum. Thanking to the method developed was possible to optimise and personalize Sanraku-en protocol.

04-11Quality assessment of Japanese Biofango through tensiometric approach by TVS mud index / 日本温泉気候物理医学会雑誌

<b>Introduction: </b>Sanraku-en spa started to purpose the “Shogawa biofangotherapy” using hot spring water and different kind of clays of natural origin (called Biofango) matured for three weeks during which bacterial flora develop with production of constitutive elements having therapeutic properties [4]. In collaboration with Ascendant Co. Ltd was ideated and developed the production of the first japanese Biofango supported by Toho University, University of Science and Technology of Hokuriku, and University of Hishigawa opening at new perspective in mudtherapy for therapeutic use in Japan (Biofango project).<BR><b>Objectives: </b>The goal was the assessing the surface energy of Biofango by TVS modelling and TVS mud index ^{1, 2)}. Tensiometric analyses of Biofango mixtures were performed at Sanraku-en spas centre (Tonami-Japan) and its quality control has been carried out in the Permanent Thermal Observatory (OTP) of University of Padova. Organic compounds analysis (TOC) were performed in the Department of Pharmaceutical and Pharmacological Sciences of University of Padova (Italy).<BR><b>Materials and Methods: </b>Biofango was prepared using KomatsuClay, MotoyamaClay, WakuraDiatomite and KasaokaBentonite. Pre-test mixtures were K01 (Bentonite 1.75, KomatsuClay 0.5, Diatomite 0.25) and M01 (Bentonite 1.75 MotoyamaClay 0.5 Diatomite 0.25). After were prepared K02 (Bentonite 1.25, Kaolinite 1.5, Diatomite 0.25) and A01 (KasaokaBentonite 1.25, Kaolinite 0.5, Diatomite 0.25) mixtures. Final Biofangos were BFM+0%Dolomite, MAT1+10%Dolomite, MAT2+18%Dolomite, and MAT3+35%Dolomite were analysed by XRF/XRD. TOC analyses were performed on a Perkin-Elmer-2400 analyser with Perkin-Elmer-AD-4 autobalance and tensiometric investigations were performed by DSA 10 (Krüss) tensiometer employing (a) PFPE, Fomblin HC/OH-1000, diiodomethane, glycerine as liquid tests and (b) Owens-Wendt³⁾ mathematical model to convert contact angles in surface enery parameters. <BR><b>Results: </b>XRD analyses of Biofango demonstrating presence of Kaolinite-Quartz-Feldspar in Komatsu Clay, Kaolinite-Quartz-Feldspar-Carbon in MotoyamaClay, Quartz-Montmorillonite-Feldspar-Grauconite in WakuraDiatomite, and Montmorillonite-Quartz-Feldspar-Christoballite in KasaokaBentonite while XRF showed presence of SiO₂ (KomatsuClay 53.38%, MotoyamaClay 48.86%, WakuraDiatomite 78.20%, KasaokaBentonite 66.01%) and Al₂O₃ (KomatsuClay 53.38%, MotoyamaClay 48.86%, WakuraDiatomite 78.20%, KasaokaBentonite 66.01%) as principal elements. TOC analyses demonstrated an increase of C% with production of CO₂ in relation to the amount of Dolomite in Biofango mixtures (MAT1=1.26%, MAT2=2.36%, MAT3=3.29%). Tensiometric investigations showed a correlation between C% and dispersed components (DC) measured on Biofango mixtures (MAT1=3.0 mN/m, MAT2=2.1 mN/m, MAT3=2.0 mN/m) and between C% and TVS mud index levels (MAT1=68.7 mN/m eq., MAT2=74.06 mN/m eq., MAT3=75.9 mN/m eq.).<BR><b>Conclusions:</b> Tensiometric investigations of Biofango consented to determine the correlations between chemico-mineralogical data and surface energy parameters. Thanking to the high sensitivity of TVS mud index used as integrated tensiometric marker was possible to determine directly and in a non invasive way the quality of Biofango mixtures opening at new perspective in their monitoring and control before their employment in mudtherapy in Japanese spas after their maturation process.

04-11 Quality assessment of Japanese Biofango through tensiometric approach by TVS mud index / 日本温泉気候物理医学会雑誌

Introduction: Sanraku-en spa started to purpose the “Shogawa biofangotherapy” using hot spring water and different kind of clays of natural origin (called Biofango) matured for three weeks during which bacterial flora develop with production of constitutive elements having therapeutic properties [4]. In collaboration with Ascendant Co. Ltd was ideated and developed the production of the first japanese Biofango supported by Toho University, University of Science and Technology of Hokuriku, and University of Hishigawa opening at new perspective in mudtherapy for therapeutic use in Japan (Biofango project). Objectives: The goal was the assessing the surface energy of Biofango by TVS modelling and TVS mud index 1, 2). Tensiometric analyses of Biofango mixtures were performed at Sanraku-en spas centre (Tonami-Japan) and its quality control has been carried out in the Permanent Thermal Observatory (OTP) of University of Padova. Organic compounds analysis (TOC) were performed in the Department of Pharmaceutical and Pharmacological Sciences of University of Padova (Italy). Materials and Methods: Biofango was prepared using KomatsuClay, MotoyamaClay, WakuraDiatomite and KasaokaBentonite. Pre-test mixtures were K01 (Bentonite 1.75, KomatsuClay 0.5, Diatomite 0.25) and M01 (Bentonite 1.75 MotoyamaClay 0.5 Diatomite 0.25). After were prepared K02 (Bentonite 1.25, Kaolinite 1.5, Diatomite 0.25) and A01 (KasaokaBentonite 1.25, Kaolinite 0.5, Diatomite 0.25) mixtures. Final Biofangos were BFM+0%Dolomite, MAT1+10%Dolomite, MAT2+18%Dolomite, and MAT3+35%Dolomite were analysed by XRF/XRD. TOC analyses were performed on a Perkin-Elmer-2400 analyser with Perkin-Elmer-AD-4 autobalance and tensiometric investigations were performed by DSA 10 (Krüss) tensiometer employing (a) PFPE, Fomblin HC/OH-1000, diiodomethane, glycerine as liquid tests and (b) Owens-Wendt3) mathematical model to convert contact angles in surface enery parameters. Results: XRD analyses of Biofango demonstrating presence of Kaolinite-Quartz-Feldspar in Komatsu Clay, Kaolinite-Quartz-Feldspar-Carbon in MotoyamaClay, Quartz-Montmorillonite-Feldspar-Grauconite in WakuraDiatomite, and Montmorillonite-Quartz-Feldspar-Christoballite in KasaokaBentonite while XRF showed presence of SiO2 (KomatsuClay 53.38%, MotoyamaClay 48.86%, WakuraDiatomite 78.20%, KasaokaBentonite 66.01%) and Al2O3 (KomatsuClay 53.38%, MotoyamaClay 48.86%, WakuraDiatomite 78.20%, KasaokaBentonite 66.01%) as principal elements. TOC analyses demonstrated an increase of C% with production of CO2 in relation to the amount of Dolomite in Biofango mixtures (MAT1=1.26%, MAT2=2.36%, MAT3=3.29%). Tensiometric investigations showed a correlation between C% and dispersed components (DC) measured on Biofango mixtures (MAT1=3.0 mN/m, MAT2=2.1 mN/m, MAT3=2.0 mN/m) and between C% and TVS mud index levels (MAT1=68.7 mN/m eq., MAT2=74.06 mN/m eq., MAT3=75.9 mN/m eq.). Conclusions: Tensiometric investigations of Biofango consented to determine the correlations between chemico-mineralogical data and surface energy parameters. Thanking to the high sensitivity of TVS mud index used as integrated tensiometric marker was possible to determine directly and in a non invasive way the quality of Biofango mixtures opening at new perspective in their monitoring and control before their employment in mudtherapy in Japanese spas after their maturation process.

04-3 Development of a method for quality assessment of Japanese Biofango mudtherapy protocol by tensiometric approach / 日本温泉気候物理医学会雑誌

Introduction: The link between the hydration state and the functional effects of formulations and natural systems is been demonstrated by several studies. Measurement of skin hydration has been used to assess barrier function integrity in vivo and stratum corneum (SC), hydration may increase after the topical application of natural or formulate systems. Thermal muds have great hydration properties thanking at its high water contain due to presence of clays minerals1). Our work was focused on the evaluation of skin’s hydration potentialities of Japanese Biofango®. Objectives: Mudtherapy increase the hydration state of skin and modify its selective permeability favouring the permeation of therapeutic substances product by maturation process. Our goal was the development of a tensiometric model for assessment and optimisation of Japanese Biofango mudtherapy protocol by the evaluation of skin hydration measuring water contact angles on skin surface2). Our work was performed at Sanraku-en spas centre (Tonami-Japan). Materials and Methods: Biofango was constituted by Kunigel, Kaolinite, and WakuraDiatomite contains montmorillonite. BFM mixture was prepared and collected from Sanraku-en maturation plant. Samples were stored at -25°C. Skin’s hydration state analysis were performed before and after treatment by contact angle method (CA) using DSA 2-Kruss Dynamic Tenskinmeter3) water as liquid test and accordingly to traditional Sanraku-en/Biofango protocol. Four subjects with differents age, sex and weights (KS, YS, MO, and KM) were considered as test. Sanraku-en mudtherapy protocol’s steps were (a) first blood pressure measurement, (b) water’s CA measurements on left and right arm before treatment, (c) thermal bath (8’), (d) water’s CA measurements, (e) BFM mudtherapy (20’), (f) water’s CA measurements on polish skin, (g) shower (3’), (h) water’s CA measurements, and (i) final blood pressure measurement. Results: CA of water on subject KS skin showed elevated levels before (CAt0>89.5 deg) and after (CAtf>89.5 deg) treatment. YS subject showed increases of skin hydration after treatment (CAt0>89.5 deg, CAtf=40.27 deg), MO showed more increase of skin hydration than YS (CAt0>89.5 deg, CAtf=20.12 deg), and KM (CAt0>89.5 deg, CAtf=41.30 deg) showed an hydration state similar to YS. Regarding YS subject, we optimized its mudtherapy by the inversion of bath (CAt0>89.5 deg, CAtf>89.5 deg) with mudtherapy phases. As results a major increase of hydration state respect normal protocol (CAt0>89.5 deg, CAtf =30.4 deg) reflecting on skin moisturize after bath phase also (CAt0>89.5 deg, CAtf=87.43 deg). Conclusions: Analyses of hydration state of skin by measurement of water’s contact angles consented to evaluate the capability of Biofango BFM to modify the selective permeability of stratum corneum. Thanking to the method developed was possible to optimise and personalize Sanraku-en protocol.